|
KMID : 0364820140500030173
|
|
Korean Journal of Microbiology
2014 Volume.50 No. 3 p.173 ~ p.178
|
|
|
Development of a Quantitative Induction Method for Chondromyces crocatus Fruiting Body Formation
|
|
Lee Cha-Yul
Shin Hye-Jin
Cho Kyung-Yun
|
|
|
Abstract
|
|
|
We have developed a method for the preparation of dispersed cell suspensions of Chondromyces crocatus, which is
essential for quantitative studies of fruiting body formation. Cells of C. crocatus have a tendency to aggregate in
liquid, hindering quantitative studies. However, cells grown on casitone-yeast extract agar plates, containing 3%
agar, allowed the preparation of well-dispersed cell suspensions. Cell suspensions at a concentration of 2 × 108
cells/ml, obtained by using this method, developed typical C. crocatus fruiting bodies when placed as 20 μl spots on
agar plates with no nutrient supplementation. The addition of nutrients such as casitone altered or inhibited fruiting
body formation. Fruiting body branch formation increased with increasing agar content. Under optimum conditions,
the formation of fruiting body structure in C. crocatus KYC2823 was completed within 24 h.
|
|
|
KEYWORD
|
|
|
Chondromyces crocatus, fruiting body, myxobacteria
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|